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. 2017 Apr 7;8(34):56351–56363. doi: 10.18632/oncotarget.16912

Figure 2. Effect of Baicalein on Akt and ERK1/2 phosphorylation and NEDD9 expression in pancreatic cancer cells.

Figure 2

(A) After BxPC-3 and PANC-1 were treated with different concentrations of Baicalein (25, 50, 75, and 100 μM) for 72 h, the protein levels of PDK1, Akt, and ERK1/2, as well as the phosphorylation levels of Akt on T-308 and S-473, and that of ERK1/2 (p-ERK1/2) were examined. β-actin was used as an inner control. (B) BxPC-3 and PANC-1 were treated with different concentrations of Baicalein (25, 50, 75, and 100 μM). DMSO was used as control. After treatment for 48 h, the mRNA level of NEDD9 was detected by qRT-PCR. ** p < 0.01, ***p < 0.001. (C) After treatment for 72 h, the protein level of NEDD9 was detected by western blot.