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. 2017 Apr 7;8(34):56351–56363. doi: 10.18632/oncotarget.16912

Figure 5. Effect of NEDD9 overexpression on Baicalein-treated pancreatic cancer cell growth and invasion.

Figure 5

(A) After treated with 50 μM Baicalein for 48 h, BxPC-3 and PANC-1 cells were transfected with the expression or empty vector for another 48 h. The viable cells were counted, and the histograms represent the statistical results. (B) Cell migration was tested by the Transwell assay and the histograms represent the statistical results. (C) Cell apoptosis was studied by flow cytometry analysis of Annexin V-FITC and PI double staining. (D) After treated with 50 μM Baicalein for 48 h, BxPC-3 and PANC-1 cells were transfected with the NEDD9 expression vector or empty vector for another 48 h. The protein levels of PDK1, as well as the phosphorylation levels of Akt on T-308 and S-473 and ERK were examined. **p < 0.01, ***p < 0.001.