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. 2017 Apr 10;8(34):56443–56459. doi: 10.18632/oncotarget.16992

Figure 3. Western blotting analysis of ERβ and MMP-2 at different time points and different concentrations.

Figure 3

(A-B) Western blotting analysis of ERα, ERβ, MMP-1, MMP-2 and MMP-9 expression in cultured NSCLC cell lines (A549 and H1793) and breast cancer cell lines (MCF-7). (C-D) The synchronized cells were treated with E2 at different concentrations (0 nM, 0.1 nM, 1 nM, 10 nM and 100 nM) for 2 days. The protein expression of ERβ and MMP-2 was analyzed using Western blot. The data represent mean ± SEM from three different experiments. E2 stimulated both ERβ and MMP-2 response in A549 cell lines in a dose-dependent manner. (E-F) The synchronized cells were treated with E2 at different time points (0 h, 1 h, 6 h, 12 h, 24 h and 48 h) at concentrations of 10 nM. The protein expression of ERβ and MMP-2 was analyzed using Western blot. The data represent mean ± SEM from three different experiments. The data represent mean ± SEM from three different experiments. E2 stimulated both ERβ and MMP-2 response in A549 cell lines in a time-dependent manner. GAPDH was used as a control.