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. 2017 Apr 10;8(34):56443–56459. doi: 10.18632/oncotarget.16992

Figure 6. pAKT inhibitor(LY294002) and p-p38MAPK inhibitor(SB203580) significantly suppressed ERβ and MMP-2 expression in NSCLC cell lines.

Figure 6

(A) Western blot analysis of protein expression of ERβ, MMP-2, p-p38MAPK, p38MAPK, pAKT and tAKT in NSCLC cell line A549 when treating with DMSO or E2 (10 nM), E2+LY294002 (10 nM), LY294002 (10 nM), E2+SB203580 (10 nM) and SB203590 (10 nM) for 48 h. (B) the detection of optical density. GAPDH was used as a control. All data are shown as the mean ± SD. Results represent three independent experiments. Student's t-test was carried out (*p < 0.05 vs. control group, #p < 0.05 vs. E2 group).