Figure 8. Effects of 17-DMAG on the growth of AGS cells xenografted in nude mice.
After 17-DMAG (10 mg/kg/day) was administered intraperitoneally 3 times a week, mice were euthanized, and their tumors were collected. (A) Morphological images of mice with xenografted AGS cells and tumors in each group. Images of tumors before and after necropsy show that tumor shrinkage was more prominent in mice treated with 17-DMAG than in control mice (P < 0.05). (B) Final tumor weight at 4 weeks. A considerably greater reduction in tumor weight was observed in mice treated with 17-DMAG than in control mice (P < 0.05). (C) Tumor volumes were calculated according to the formula: volume = length × width2 × 0.5236. Note that a significant reduction in tumor volumes was detected in mice treated with 17-DMAG compared with control mice (P < 0.05). (D) Body weight changes during the experiment. The average body weight of mice in each group did not vary significantly over the course of the experiment. (E) Immunohistochemical stains demonstrating that 17-DMAG treated group exhibited the negative rates of PCNA and survivin expression and the positive rates of c-Caspase 9 expression. (F) Western blot analysis showing that 17-DMAG treated group exhibited significantly lesser expression of antioxidant enzymes than did the control group. c-Cas3: cleaved-caspase 3, GPx, Glutathione peroxidase; MnSOD, Manganase superoxide dismutase; PCNA, Proliferating cell nuclear antigen. *P < 0.05.