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. 2017 Apr 10;8(34):56490–56505. doi: 10.18632/oncotarget.17009

Figure 3. TTSPs proteolytically modify PDGFRs and TrkA.

Figure 3

The cDNA coding for a carboxyl-terminally HA-tagged receptor was co-transfected in HEK293 cells with an empty plasmid (pcDNA3, Invitrogen) (Control, Lane 1), or the cDNA coding for human matriptase (Mat, Lane 2), protease-dead matriptase (Mat-Mut, Lane 3), Hepsin (Lane 4), protease-dead hepsin (Hepsin-Mut, Lane 5), mouse TMPRSS6 (Lane 6), protease-dead mouse TMPRSS6 (TMPRSS6-Mut, Lane 7), human Prostasin (Lane 8), a GPI-anchored matriptase (GPI-Mat, Lane 9) and protease-dead GPI-anchored matriptase (GPI-Mat-Mut). Twenty micrograms of total cell lysate from each sample were resolved on SDS-PAGE and western-blotted with an anti-HA antibody or an anti-β-tubulin antibody. The p185 PDGFRα (A), p185 PDGFRβ (B) or p140 TrkA (C) is indicated by the arrows, and the CTFs are indicated by the red arrowheads.