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. 2017 Apr 10;8(34):56490–56505. doi: 10.18632/oncotarget.17009

Figure 7. Matriptase knockout enhances PMA-induced Her2 turnover in SKBR3 human breast cancer cells.

Figure 7

An equal amount of total protein lysate (40 μg) from the SKBR3 (Lanes 1 and 2), SKBR3/CC-SCR (Lanes 3 and 4), and SKBR3/CC-ST14 (Lanes 5 and 6) cells was used for western blot analysis of Her2 (Top Panel), matriptase (Mat, Second Panel from the Top), and PKCα (Third Panel from the Top). Cells represented by samples in Lane 2, 4, and 6 were treated with 1 μM PMA for 16 hours, and those in Lanes 1, 3, and 5 with the vehicle control (DMSO). A GAPDH western blot (Bottom Panel) was performed as a loading control.