Figure 4. Validation of candidate miRNAs.
(A) Venn diagram representing the number of miRNAs that were found to be down-regulated in ALK+ group (or up-regulated in ALK- group). Filtering criteria for prioritizing miRNAs for validation and functional analyses are described in the results. (B) Validation analysis of miRNAs expression was performed by qPCR and data were box-plotted using BoxPlotR. p-value < 0.05 (*); p-value < 0.01 (**). (C) miR-424-5p/ALK 3′-UTR alignment as predicted by MicroRNA.org. Sequence starts at 321 bp of ALK 3′-UTR. mirSVR score:-0.9220; PhastCons score: 0.5638.