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. 2017 Apr 11;8(34):56518–56532. doi: 10.18632/oncotarget.17033

Figure 6. Impairment of ALK expression and cell viability assay.

Figure 6

(A) Western blot analysis of ALK after transient transfection with miR-424-5p and miR-503-5p mimics. SKNSH cells revealed a marked decreasing of ALK after 72 hours. NB1 cells showed a similar ALK reduction after 48 hours, although its expression restored at 72 hours (data not shown). Quantification of bands was performed by ImageJ software and experiments were repeated at least once with consistent results. (B) MTT test to assess cell viability after transient transfection with miR-424-5p and miR-503-5p mimics at 48 and 72 hours in two ALK+ cell lines (SKNSH and NB1) and one ALK- cell line (SKNAS). p-value < 0.05 (*); p-value < 0.01 (**).