Figure 1. Effect of ING5 on aggressive phenotypes of glioma cells.

After transfection of pEGFP-N1-ING5, ING5-overexpressing clones were selected by realtime PCR (A) and western blot (B). ING5 overexpression suppressed proliferation by MTT assay (C), glucolysis and mitochondrial respiration by cellular energy metabolism assay (D), differentiation by ALP activity (E), and induced G₂/M arrest by PI staining (F), apoptosis by Annexin-V staining (G) in U87 cells. ING5 decreased the ability of U87 cells to migrate and invade, evidenced by wound healing (H) and transwell (I) assays. ING5 overexpression also promoted the U87 cell senescence by β-galactosidase staining (J), * p < 0.05.