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. 2017 Apr 28;8(34):56582–56597. doi: 10.18632/oncotarget.17508

Figure 8. Fluorouracil treatment induces mitochondrial fission and apoptosis, and shows a similar trend of changes in MTP18 expression as doxorubicin exposure.

Figure 8

(A) and (B) Fluorouracil (5-FU) induces mitochondrial fission in gastric cancer cells. (C) and (D) 5-FU induces apoptosis in gastric cancer cells. The cells were stimulated with 5-FU (4μmol/L) in AGS cell lines (A) and (C) or (10 μmol/L) in NCI-N87 cell lines (B) and (D) and then harvested at the indicated time for mitochondrial fission and apoptosis analysis. Percentage of apoptosis was analyzed by DNA fragmentation using the cell death detection ELISA. Data were expressed as the mean±SEM of three independent experiments. **P<0.01, ***P<0.001, and ****P<0.0001 compared to non-treated control. (E) and (F) 5-FU down regulates MTP18 expression in gastric cancer cells. (E) and (F) Show kinetics of MTP18 expression. AGS (E) and NCI-N87 (F) cell lines were stimulated with indicated dose of 5-FU and then harvested at the indicated time for immunoblotting. β-actin or tubulin served as a loading control.