Figure 4. Wip1 knockdown inhibited the migration ability of ICC cells in vitro.
Representative images of the migrate ICC-9810 and SSP25 cells in Matrigel assays were taken at ×200 magnification (A,B,D,E) Numbers of the invaded ICC-9810 and SSP25 cells per microscopic field (C,F). In a wound healing assay, ICC-9810 and SSP25 cells in the Wip1-shRNA group exhibited decreased migration ability compared with control group (G) Cells were monitored every 24 h for 2 days to evaluate the rate of migration into the scratched area (H).*P<0.05.