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. 2017 Jun 16;8(34):57012–57023. doi: 10.18632/oncotarget.18505

Figure 4. EGFR is a direct target of miR-107-5p.

Figure 4

(A) Identification of the candidate target of miR-107-5p by bioinformatics. Blue: the predicted targets generated by targetscan. Yellow: the genes involved in apoptosis. Green: the genes involved in EGFR signaling pathway. Pink: the genes involved in cadherin signaling pathway. (B) Schematic of the seed region of miR-107-5p in the 3′-UTR of EGFR (EGFR-3′-UTR) and the mutated 3′-UTRs (EGFR-3′-mUTR) as predicted by bioinformatics. (C) There was a significant decrease in the luciferase activity of HEK293T cells co-transfected with miR-107-5p mimic and pGL3-EGFR-3′-UTR. (D) Gene expressions were measured by western blot in A549 and HCC827 cells transfected with NC and miR-107-5p mimic. All experiments were repeated in triplicate (*P < 0.05, ** P<0.01, *** P<0.001).