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. 2017 Jul 4;8(34):57058–57071. doi: 10.18632/oncotarget.18983

Figure 4. Effect of daurinol on MMP2, MMP9, and uPA expression and activity.

Figure 4

(A) Daurinol-treated MDA-MB-231 and A549 cancer cells CM was subjected to zymograph assay for analysis of MMP2, MMP9, and uPA activities. Equal volumes of serum-free culture medium were obtained from control cells or cells that had been treated with indicated dose of daurinol for 48 hours. Culture media were concentrated with Amicon Ultra-4 centrifugal device obtained from Millipore and loaded onto gels. The proteolytic activity of MMP-2/MMP-9 and uPA was assessed by SDS-PAGE using zymogram gels containing 0.1% (m/v) gelatin and fibrinogen/plasminogen, respectively. (B) Semi-quantitative RT-PCR was performed for analysis of MMP2, MMP9, and uPA expression. After 48 h of treatment with different concentrations of daurinol in MDA-MB-231 and A549 cancer cells.