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. 2017 Jul 6;8(34):57072–57088. doi: 10.18632/oncotarget.19046

Figure 6. Prolactinomas of lacDrd2KO female mice have an active Notch system.

Figure 6

(A–C) Gene expression determined by qRT-PCR in pituitaries of lacDrd2KO and Drd2loxP/loxP counterparts normalized to Gapdh and expressed as percentage of change of the Drd2loxP/loxP pituitaries considered 100%. Bars show mean values. Notch1 *p = 0.0431, Notch3 *p = 0.0089 vs. Drd2loxp/loxp; Notch2, 4 NS, n = 7, 5 for lacDrd2KO and Drd2loxP/loxP mice, respectively (A). Notch ligands Dll1 and Jagged1 NS (B), and Notch target genes Hes1 (*p = 0.0106 lacDrd2KO vs. Drd2loxP/loxP), Hes5 and Hey2, NS (C); n = 7, 5 for lacDrd2KO and Drd2loxP/loxP mice, respectively. (D–E) The expression of the active and membrane domains of NOTCH1-3 receptors was determined by Western blot, related to the correspondent β actin levels, and expressed as the percentage of Drd2loxP/loxP pituitary average. *p = 0.01 for NOTCH1, NOTCH2, 3 NS active domain, p =0.0604 and p = 0.0042 for NOTCH2 and NOTCH3 membrane domains, respectively for lacDrd2KO vs Drd2loxP/loxP mice. n = 4, 3–4 for lacDrd2KO and Drd2loxP/loxP. Representative Western blots showing the active and membrane domains of NOTCH receptors in lacDrd2KO and Drd2loxP/loxP (F). Representative microphotographies of NOTCH1-3 and HES1 Immunohistochemistry performed in lacDrd2KO and Drd2loxP/loxP mice (G).