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. 2017 Jul 11;8(34):57174–57186. doi: 10.18632/oncotarget.19170

Figure 2. miR-223-3p induced CAL27 proliferation.

Figure 2

(A) Characterization of CAL27 miR-223-3p cells. Total RNA was isolated from CAL27-Luci (named CAL27) and CAL27-Luci miR-223-3p (named CAL27 miR-223-3p) cells and RT-PCR analysis was performed. We showed that miR223 is overexpressed in transfected cells. Rnu19 RNA was used as internal control; Cp value for CAL27 cells was 32.60. (B, C) Expression of miR-223-3p in CAL27 cells improved cell proliferation (n = 4), whereas it had no effect on cell migration. (C) Confluent monolayers were scratched with a yellow tip and cell migration was expressed as the percentage of wound recovery (n = 3). Data are presented as mean ± sem.