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. 2017 Jul 11;8(34):57174–57186. doi: 10.18632/oncotarget.19170

Table 1. Association of miR-223-3p levels with neutrophil infiltrate and CD31 expression.

Neutrophil P-value MiR-223-3p staining P-value CD31 expression P-value
Low Medium + High Low Medium + High % high exp Negative Positive % high exp
Control 6 2 0.042* 6 2 0.05* 8 0 0.03*
Early-stage T 2 20 0.001# 2 20 90 0.001# 17 5 21 0.2#
Advanced-stage T 9 4 0.999 ± 10 3 23 0.99 ± 3 10 77 0.001 ±

Statistical analysis: Fisher's exact test. *Control vs. Tumors. #Control vs. Early-stage Tumors. ± Control vs. Advanced-stage Tumors.

Quantification of neutrophil infiltrate, miR-223-3p staining and CD31 expression from a control group made of 8 healthy people, a group made of 22 HNSCC patients with early-stage (T1 or T2) tumors and a group made of 13 patients with advanced-stage (T3 and T4) tumors was performed by a trained pathologist as following. A sample was considered to display low expression if the percentage of positive cells was from 0 to 10%, median expression (med) if the percentage is between 10 to 50% and high expression if more than 50% of the cells were positive. Negative scoring for CD31 staining corresponds to less than 30% positive cells. Three random fields per tumor were analyzed. This contingency table highlights a correlation between the presence of neutrophils and the expression of miR-223-3p and an inverse correlation between the presence of miR-223-3p and the expression of CD31.