Table 1. Comparison of fluorophore and Ab-F properties.

Fluorophore	Molecular Weight Daltons	ε (Fluorophore) cm−1 M−1	λEx max nm	Degree of Labeling (DOL)	Injected Dose (mg/kg)
BODIPY630	660.5	101000	625	1.3	5.8
VT645	1393	210000	643	2.3	3.3
AF647	1250	239000	650	2.5	3.0
VT680	1856	210000	668	2.4	3.1
AF680	1150	184000	679	3.3	2.3
AF750	1300	290000	752	2.3	3.3
IR800Dye	1166	240000	778	1.4	5
DyLight800	1050	270000	770	2.5	N/A

For each fluorophore, the molecular weight (Da), emission coefficients (ε: cm⁻¹ M⁻¹) and absorption maxima (ex/em: nm) are provided. The degree of labeling obtained for each Ab-F and respective in vivo doses (mg/kg) that equate to a 2 nmol dose of fluorophore are shown in the last two columns.