Table 2. SEC-HPLC analysis of Ab-F conjugate.
| Label | RT (min) | %Area |
|---|---|---|
| Unlabeled Ab | 15.7 | 95.9 |
| BOD630 | 15.4 | 89.0 |
| VT645 | 15.5 | 95.3 |
| AF647 | 15.6 | 95.8 |
| VT680 | 15.5 | 95.8 |
| AF680 | 15.7 | 87.3 |
| AF750 | 15.6 | 95.3 |
| IRDye800 | 15.3 | 89.8 |
| DyLight800 | 19.5 | 46.9 |
The stability of Ab-F conjugates was evaluated using SEC-HPLC analysis. The retention time (RT) and the Area (%) of Ab-F conjugates was compared to the RT of the unconjugated Ab (15.7 min). All Ab-F conjugates showed an RT between 15.3–15.7, except DyLight800, whose major peak had an RT of 19.5 min.