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. 2017 Jul 18;8(34):57302–57315. doi: 10.18632/oncotarget.19328

Figure 6. CTDSPL and CTDSPL2 promote cellular migration in chick embryo fibroblasts.

Figure 6

(A) A scratch assay was performed to monitor cell migration. Representative images of scratches at time 0 and at 8 hours are shown. (B) Quantification of wound closure. On average, uninfected CEF and CEF infected with empty viral vector exhibit approximately 5% wound closure after 8 hours. Cells expressing truncated CTDSPL2 (TR2) exhibited significantly faster cellular migration rates with 18% wound closure on average after 8 hours (p < 0.0001). Cells expressing full length CTDSPL2 (FL2) had the fastest migration with 25% wound closure at the final time point (p < 0.0001). Cells overexpressing CTDSPL truncated and full-length (TR1, FL1) transcripts had intermediate phenotypes with approximately 15-20% migration.