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. 2017 Jul 18;8(34):57302–57315. doi: 10.18632/oncotarget.19328

Figure 8. Overexpression of truncated CTDSPL and CTDSPL2 promotes immortalization of primary cells in culture.

Figure 8

Proliferation data shown is from day 42 to day 53 after infection. (A) Representative growth curve of CEF cells infected with virus, expressing truncated or full length CTDSPL or CTDSPL2. (B) Average growth curve of three biological replicates. CEF cells as well as cells infected with empty virus stop proliferating at later time points. Cells overexpressing full length CTDSPL or CTDSPL2 (FL1, FL2) proliferate less on average than uninfected CEFs. However, cells overexpressing truncated viral fusion transcripts of either CTDSPL or CTDSPL2 (TR1, TR2) exhibit significantly higher proliferation at later time points indicating that they may be promoting immortalization.