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. 2017 May 11;8(34):57907–57933. doi: 10.18632/oncotarget.17826

Figure 6. The effect of C1orf64 on the AR-mediated repression of PGR and AR reporter activity.

Figure 6

(A) qRT-PCR to assess the effects of E2 and DHT on PGR expression in T-47D cell line. CTL: solvents only, E2: E2 at 10 nM, DHT: DHT at 10 nM, E2 + DHT: each at 10 nM. *, p< 0.01 is for each treatment group vs. CTL. (B) qRT-PCR to measure the effect of C1orf64 silencing using siRNA-D1 (D1) and siRNA-D2 (D2) on PGR expression in T-47D cells. CTL: control-siRNA. *, p< 0.01 is for CTL vs. D1 or D2 (C) qRT-PCR to examine the combined effects of DHT (10 nM) treatment and C1orf64 silencing on PGR expression. CTL: CTL-siRNA, *, p< 0.01 is for CTL vs. CTL-DHT, and **, p< 0.03 is for CTL-DHT vs. siRNA-D1 + DHT or siRNA-D2 + DHT. (D) qRT-PCR to assess the effect of C1orf64 overexpression on PGR expression. CTL-VEC: Control vector, E2/DHT: E2 (10 nM) + DHT (10 nM), C1orf64: C1orf64 overexpression. *, p< 0.01 is for CTL-VEC vs. CTL-VEC + E2/DHT or C1orf64 vs. C1orf64 + E2/DHT. (E-F) AR reporter assays in T-47D (E) and MFM-223 (F) cell lines. Relative response ratio (RRR) is shown for each reporter assay. Neg-Rep/Vec: negative control for reporter + empty plasmid, Neg-Rep/C1orf64: negative control for reporter + C1orf64 construct, Pos-Rep/Vec: AR reporter + empty plasmid, and Pos-Rep/C1orf64: AR reporter + C1orf64 construct. *p<0.01 is for Pos-Rep/Vec vs. Neg-Rep/Vec, **p< 0.01 is for Pos-Rep/C1orf64 vs. Pos-Rep/Vec or Neg-Rep/C1orf64. Error Bars: ± 2SEM.