Figure 7. The effect of C1orf64 on AR promoter binding and the interaction between C1orf64 and AR.

(A) Fold enrichments of ChIP for PIP promoter performed with an AR antibody (AR-Ab) following the transfections of control (CT)-siRNA or C1orf64-siRNA (Duplex 1) in T-47D cells. AR binding to each promoter region (A1-A4) was assessed. Amplification of 1% of input chromatin was used as the input control and a non-specific antibody served as negative control (dashed-line). *, p< 0.03 is for C1orf64-siRNA vs. CT-siRNA in A1 and A2. Error Bars: ± 2SEM. (B) Co-immunprecipitation assays (Co-IP) to investigate the interaction between endogenous C1orf64 and AR in T-47D and MFM-223 cell lines. AR-IP was performed using an AR antibody and control experiments were conducted with a non-specific rabbit IgG. Immunoblot analysis were carried out on IP supernatants using C1orf64 and AR antibodies. Input was assessed with AR immunoblotting on 5% of lysates collected before each IP. All co-IP experiments were performed in three replicates.