Figure 8. An interaction between C1orf64 and 14-3-3.

(A and B) C1orf64 protein sequence was analyzed using Scansite software to identify binding sites for regulatory motifs. Motif scan was carried out with a high stringency to detect the best 0.2% of all sites. (A) Shows each motif, sequence score with percentile, sequence of motif, and surface accessibility of the predicated site. (B) Depicts the motif sites within C1orf64 sequence. (C) Co-immunprecipitation assays to examine the interaction between endogenous 14-3-3 and C1orf64 as well as with AR in T-47D and MFM-223 cell lines. IP assays were performed using a 14-3-3 antibody and control experiments were conducted with a non-specific rabbit IgG. Immunoblot analysis were carried out on IP supernatants using 14-3-3, C1orf64, and AR antibodies. Input was assessed with 14-3-3 immunoblotting on 5% of lysates. All co-IP experiments were performed in three replicates.