Table 1.
Expression of programmed death ligand 1 (PD-L1) in Kaposi's sarcoma (KS).
Patient | Age | Years on cART | Years HIV positive | Years since first KS diagnosed | Peripheral blood CD4+ count (cells/mm3) | Peripheral blood CD8+ count (cells/mm3) | KS case or normal skin control | Amount of tumor | PD-L1 tumor-cell expression | PD-L1-expressing macrophages | Tissue CD8+ count (cells/mm2) | KS Staging (ACTG criteria) | KS grade | Plasma HHV8 viral load copies/mL |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
S926 | 53 | 2.8 | 26.3 | 1.3 | 499 | 830 | Case | Small | 0 | Absent | 76 | T1I0S0 | Patch | 42400 |
Control | Absent | 0 | Absent | 24 | ||||||||||
M907 | 59 | 16.2 | 16.3 | 13.7 | 111 | 1101 | Case | Small | 0 | Rare | 106 | T1I0S0 | Plaque | 0 |
Control | Absent | 0 | Absent | 32 | ||||||||||
M515 | 54 | 10.5 | 13.2 | 13.2 | 913 | 482 | Case | Abundant | 1+ | Numerous | 292 | T1I0S0 | Nodular | 0 |
Control | Absent | 0 | Absent | 15 | ||||||||||
M610 | 49 | 9.0 | 9.9 | 3.0 | 574 | 820 | Case | Moderate | 1+ | Rare | 44 | T1I0S0 | Nodular | 0 |
Control | Absent | 0 | Absent | 5 | ||||||||||
L292 | 37 | 1.2 | 1.2 | 1.2 | 298 | 1497 | Case | Abundant | 1+ | Moderate | 225 | T1I1S1 | Nodular | 0 |
Control* | Focal | 1+ (focal) | Rare | 111 | ||||||||||
S371 | 58 | 8.3 | 8.3 | 8.3 | 677 | 961 | Case | Focal | 0 | Absent | 36 | T1I0S1 | Lymphangiectatic | 0 |
Control | Absent | 0 | Absent | 15 | ||||||||||
L432 | 45 | 0.8 | 4.4 | 0.8 | 459 | 709 | Case | Small | 1+ (focal) | Rare | 190 | T0I0S0 | Plaque | 1650 |
Control | Absent | 0 | Absent | 13 | ||||||||||
R366 | 39 | 11.0 | 12.0 | 11.1 | 1200 | 961 | Case | Small | 0 | Absent | 72 | T0I0S0 | Patch | 0 |
Control | Absent | 0 | Absent | 7 | ||||||||||
G099 | 43 | 10.5 | 13.4 | 0.1 | 1219 | 732 | Case | Abundant | 1 | Rare | 156 | T0I0S0 | Plaque | 0 |
Control | Absent | 0 | Absent | 34 | ||||||||||
G963 | 33 | 1.0 | 2.0 | 0.9 | 661 | 738 | Case | Absent | 0 | Absent | 22 | T0I0S0 | Patch | 541 |
Control | Absent | 0 | Absent | 23 |
This table summarizes the results of histological examination of Kaposi's sarcoma (KS) biopsies (cases) and normal skin controls from 10 patients with KS despite established, successful antiretroviral therapy, undetectable plasma HIV-1 RNA load and good CD4+ T-cell counts. The amount of KS tumor in each was semi-quantitatively assessed. Expression of programmed death ligand 1 (PD-L1) by tumor-cells was judged according to a scoring system from 0 to 3+. No tumors showed expression greater than 1+ (any staining above background). The number of tumor-infiltrating PD-L1-expressing macrophages was semi-quantitatively assessed. The number of tumor-infiltrating CD8+ T cells was calculated as cells per mm2.
indicates this control biopsy contained a focal area of tumor not clinically apparent.