Figure 1.

Cd226^−/− NK cells show impaired cytotoxicity. (A) Phenotypes of wild-type (WT) NK cells (n = 3). (B) Phenotypes of Cd226^−/− NK cells (n = 3). (C) Phenotypes of B16F10 cells (n = 3). (D) Cytotoxicity of NK cells against B16F10 cells was determined by the LDH assay (n = 3, *p < 0.01). (E) To analyze exocytosis, NK and B16F10 cells were co-cultured for 2 h in the presence of anti-CD107a-FITC antibody, and CD107⁺ expression level on NK cells was determined using flow cytometry (n = 3, *p < 0.01). (F) Intracellular perforin and granzyme B protein levels of wild-type and Cd226^−/− NK cells (n = 3).