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. 2017 Jul 5;6(8):e1338994. doi: 10.1080/2162402X.2017.1338994

Figure 5.

Figure 5.

Classification of NK cells based on contact modes. (A) Unstained NK cells (unsorted WT, CD226+ WT, CD226− WT, or Cd226−/− NK cells) and calcein AM–stained B16F10 cells (transfected with CD155 siRNA or not) were imaged every 2 min from 1 h to 5 h. Representative images of the contact modes of CD226+ NK cells (arrows) showing free migration, killing contact, and non-killing contact (magnification, 200 ×; electronically zoomed; scale bars, 15 μm; n = 10 movies of 3 independent experiments per group). (B) Ratios of contacting NK cells to total NK cells (n = 1,413, 527, 521, 376, and 688 from the left). (C) Ratios of killing per total contacts (n = 279, 93, 118, 84, and 146 from the left). (D, E) Contact duration of killing and non-killing interactions between cancer cells and unsorted (n = 79), Cd226−/− (n = 191), CD226+ (n = 71), and CD226− (n = 145) NK cells. (n = 3, *p < 0.01).