Human microglia signature genes are present in MMG cells. Human healthy donor blood was used to isolate MMG from monocytes. MMG were generated in vitro by culturing CD14+ cells in the presence of M-CSF, GM-CSF, NGF-β, and CCL2 for 10–12 days. HMG cells were isolated from 90- to 145-day-old human fetal brain tissue. qPCR analysis of human microglia signature genes in MMG (n = 3) and HMG cells (n = 3) for c1q, gas6, gpr34, pros1, p2ry12, and mertk genes. Gene expression was normalized against GAPDH using ΔCt (n = 3). Microglia signature gene expression is compared between MMG cells and HMG cells derived from three independent healthy human donor bloods and fetal brain tissues, respectively. Data are presented as ± SD, n = 3; *P < 0.05