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. Author manuscript; available in PMC: 2017 Sep 11.
Published in final edited form as: J Neurovirol. 2016 Aug 18;23(1):33–46. doi: 10.1007/s13365-016-0472-1

Fig. 4.

Fig. 4

Human microglia signature genes are present in MMG cells. Human healthy donor blood was used to isolate MMG from monocytes. MMG were generated in vitro by culturing CD14+ cells in the presence of M-CSF, GM-CSF, NGF-β, and CCL2 for 10–12 days. HMG cells were isolated from 90- to 145-day-old human fetal brain tissue. qPCR analysis of human microglia signature genes in MMG (n = 3) and HMG cells (n = 3) for c1q, gas6, gpr34, pros1, p2ry12, and mertk genes. Gene expression was normalized against GAPDH using ΔCt (n = 3). Microglia signature gene expression is compared between MMG cells and HMG cells derived from three independent healthy human donor bloods and fetal brain tissues, respectively. Data are presented as ± SD, n = 3; *P < 0.05