Fig. 9.

A) DRG cultured on topographically patterned and flat substrates stained for A, B, F, G) F-actin, C, H) rictor and D, I) DNA. E) Rictor (red) and F-actin (green) staining seen throughout neurites, with maximal co-localization at growth cones (yellow). Scale bar 200 μm in magnified images, 500 μm in whole DRG image. K) Plot of normalized grey value against distance along axon from tip towards cell body in DRGs cultured on topography (K) and flat substrates (L). Quantitative analysis demonstrates peak F-actin grey value at the tip of the growth cone, peak rictor grey scale is localized just proximal to this however relative intensity change along the neurite is not as striking as that seen on grooved surfaces (mean and SEM, n = 12). Grey values normalized to the maximum detected along the whole neurite.