Table 4.
Results of global analysis of fluorescence decay kinetics on isolated proteins.
| Sample | A1 | τ1 (ps) | A2 | Τ2 (ps) | τavg (ps) |
|---|---|---|---|---|---|
| LHCSR1 CTRL pH 7.5 0.03% a-DM | 26% | 974 | 74% | 3756 | 3042 |
| LHCSR1 DEP pH 7.5 0.03% a-DM | 39% | 750 | 61% | 3123 | 2198 |
| LHCSR1 CTRL pH 5 0.03% a-DM | 44% | 634 | 56% | 4080 | 2567 |
| LHCSR1 DEP pH 5 0.03% a-DM | 52% | 551 | 48% | 2971 | 1720 |
| LHCSR1 CTRL pH 7.5 0.007% a-DM | 52% | 198 | 48% | 3446 | 1742 |
| LHCSR1 DEP pH 7.5 0.007% a-DM | 49% | 366 | 51% | 2982 | 1687 |
| LHCSR1 CTRL pH 5 0.007% a-DM | 81% | 87 | 19% | 1574 | 372 |
| LHCSR1 DEP pH 5 0.007% a-DM | 82% | 86 | 18% | 677 | 192 |
| LHCSR1 CTRL pH 7.5 0.003% a-DM | 69% | 109 | 31% | 1559 | 553 |
| LHCSR1 DEP pH 7.5 0.003% a-DM | 64% | 110 | 36% | 1175 | 495 |
| LHCSR1 CTRL pH 5 0.003% a-DM | 64% | 67 | 36% | 235 | 127 |
| LHCSR1 DEP pH 5 0.003% a-DM | 100% | 81 | — | — | 81 |
2D Streak camera maps were fitted with a bi-exponential decay function with a global analysis method. Amplitude and time constants are reported. Average fluorescence lifetimes were calculated as ΣAiτi/ΣAi.