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. 2017 Sep 11;7:11225. doi: 10.1038/s41598-017-10866-1

Table 1.

Details of primers and PCR coditions used for the amplification of KLK3 exons.

Primer Pair Primer sequences (Forward/Reverse) Annealing temperature (°C) Product size (bp)
1 GGGGGTTGTCCAGCCTCCAGCAG/GCGGGGACCTGGTGTGGGAGTG 63 404
2 GCCCCCGTGTCTTTTCAAACCC/TCCCATGCGTGTGCTCAGTAGG 65 708
3 TGCCCTTCACCCTCTCACACTG/GGGGTCAAGACTACGGGCCAGGC 63 509
4 GGTGCAGCCGGGAGCCCAGATG/CGGGGAGGTGGCATGGCTACAG 65 391
5 GGGGGTGGCTCCAGGCATTGTCC/AGGGGGTTGATAGGGGTGCTC 65 722
6 GGTGTGAGGTCCAGGGTTGCTAGG/CCACTGGGAGAAAACAACTGAAAG 65 634