Figure 6.

RNA binding activity of Rbfox family protein is crucial for its SG localization. (a) Diagram of myc-tagged deletion or mutation constructs of Rbfox3. (b) HeLa cells were transfected with indicated Rbfox3 constructs for 24 hr. Representative immunoblot with anti-myc showing the protein products from the expression plasmids. (c–f) HeLa cells were transfected with constructs shown in Fig. 6a. Immunofluorescence images of myc-Rbfox (anti-myc, red) and TIA-1 (green) proteins were visualized in untreated (Control) and sodium arsenite-treated HeLa cells (Stress). DAPI stains nuclei. Scale bar, 20 μm.