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. 2017 Sep 11;7:11211. doi: 10.1038/s41598-017-11651-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rbfox2 affects RB1 protein expression under cellular stress. (a) HeLa cells were transfected with siCon. or siRbfox2 for 36 hr. Representative immunoblot showing the effects of siRNA transfections on protein levels of Rbfox2 (green) and Gapdh (red). (b) The steady- state levels of RB1 and GAPDH mRNAs were measured by qRT-PCR using total RNA from control HeLa cells (siCon.) and Rbfox2-deficient HeLa cells (siRbfox2) treated for 40 min with or without sodium arsenite (500 μM), followed by treatment for 0–3 hr with actinomycin D (2.5 μg/ml) in arsenite-free media. *P < 0.05, comparing stressed siRbfox2 with siCon. samples. (c,d) HeLa cells transfected with siCon. or siRbfox2 for 36 hr were treated with sodium arsenite, followed by recovery for 3 and 24 hrs. Cell lysates were subjected to immunoblot analysis using the indicated antibodies. Bands were quantified via densitometry and normalized to Gapdh. Mean values ± SD (error bars) are shown for three independent experiments. *P < 0.05 (two-sided t test), comparing stressed siRbfox2 with siCon. samples.