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. 2017 Sep 11;7:11191. doi: 10.1038/s41598-017-10247-8

Figure 2.

Figure 2

KFD2-rPAc induced a much lower systemic inflammatory response after intranasal administration than KF-rPAc. Female BALB/c or C57BL/6 (WT or TLR5 KO) mice were intranasally treated with indicated amount of rPAc, KF-rPAc, or KFD2-rPAc in 30 μl of PBS or PBS alone. (a) Body weight changes of BALB/c mice after 10 μg (left panel) or 50 μg (right panel) recombinant protein administration. IL-6, KC and TNF-α in serum (b) or BALF (c) after 4 h of administration of 50 μg recombinant protein to BALB/c mice were determined by ELISA kits. IL-6, KC and TNF-α in serum (d) or BALF (e) after 4 h of administration of 50 μg recombinant protein to C57BL/6 background WT or TLR5 KO mice were determined by ELISA kits. Data are presented as mean ± SEM from 1 experiment that was repeated 3 times (n = 6 per group). *p < 0.05; **p < 0.01; and ***p < 0.001; n.s., non-significant.