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. 2017 Sep 11;7:11210. doi: 10.1038/s41598-017-10348-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sagittal section of a decalcified microcolony stained with hematoxylin and eosin. (A) Photo of a decalcified microcolony used for sagittal section as indicated by the red line. (B) Reconstruction of the general view of the stained sagittal section with hematoxylin and eosin. The nubbin is localized between the two black lines. The adjacent parts correspond to the tissue growing on the coverslip (Scale: 1 mm). (C) Histology at the nubbin’s apex (Scale: 50 μm). (D) Histology of the tissue growing on the coverslip. The asterisk indicates secreted IOM by the aboral ectoderm (Scale: 50 μm). (E) Histology of the tissue growing at the growing edge on the coverslip (Scale: 50 μm). Po: Polyp; GC: Gastrodermal canals; OE: Oral ectoderm; AE: Aboral ectoderm; Mgl: Mesoglea; Sclb: Scleroblasts; nu Sclb: Scleroblasts nuclei; IOM scl: Insoluble organic matrix of sclerites; IOM ax: Insoluble organic matrix of the axial skeleton.