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. 2017 Sep 11;7:11210. doi: 10.1038/s41598-017-10348-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Observation of growing sclerites and their cellular environment by confocal microscopy and TEM. (A-A’) Confocal images of growing sclerites labeled with anti sclerite SOM (red), and scleroblasts labeled with Phalloidin (actin, green) and DAPI (nuclei, blue). Arrows suggest the secretion of OM at the level of scleroblasts membranes. Arrowheads show junction between scleroblasts. The merged images show the cellular environment of growing sclerites, surrounded by two scleroblasts. (A) Observation of a section along the optical axis Z in a sclerite. (A’) Maximum intensity projection (MIP) of a z-stack of multiple optical sections with a 0.2 µm step between them. (B-B’) TEM images of a transversal section in a C. rubrum microcolony showing septate junctions between two scleroblasts. (B) Sclerite in the mesoglea surrounded by two scleroblasts. Yellow boxes indicate the presence of two septate junctions. (B’) Enlargement showing a septate junction between two scleroblasts. Mgl: Mesoglea; OM scl: Organic matrix of sclerite; Sclb: Scleroblasts.