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. 2017 Sep 11;7:11091. doi: 10.1038/s41598-017-11357-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cuticle thickness in resistant MRS and susceptible Kisumu measured by transmission electron microscope. Thicknesses of the whole cuticle (a) and of its various layers (b) were measured with an Image J 1.49. Box plots represent the minimum and maximum quartiles, dots the outlying data. n = the number of measures carried out on each batch of 20 mosquitoes (Kisumu & MRS). *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001 according to a Wilcoxon rank sum test.