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. 2017 Sep 11;7:11108. doi: 10.1038/s41598-017-11551-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional expression of ChR2 in C2C12 myotubes investigated by patch-clamp. (A) Inward ChR2 current recorded at a holding potential of −50 mV during a light pulse of 4 ms at various light intensities. Light pulse is schematized as a blue rectangle. (B) ChR2 current recorded as described in A represented as percentage of maximum ChR2 current recorded at 4.3 mW.mm⁻². Data are expressed as mean ± SEM (n = 6).