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. 2017 Sep 11;7:11108. doi: 10.1038/s41598-017-11551-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial light-induced calcium response properties of ChR2-GFP expressing cells. (A) Global fluorescence records (insets) in the green dotted line outlined area of a ChR2-GFP expressing myotube depending on the position of the optical stimulation square (blue squares). (B) Local fluorescence records (insets) from regions of interest inside and outside the myotube (dotted lines squares) during an optical stimulation (blue square) inside the same myotube as in A. Bar: 10 µm.