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. 2017 Sep 11;7:11242. doi: 10.1038/s41598-017-11687-y

Figure 5.

Figure 5

Cytotoxicity assays of HMS-COOH and RGD-hylin a1-HMS (pH = 7 and pH = 5.5) in Hela cells. Cellular morphology was observed by 10× inverted microscope. (A) Hela cells were treated with 25 μg/ml HMS-COOH for 24 h; (B) Hela cells were treated with 25 μg/ml RGD-hylin a1-HMS (pH = 7) for 24 h; (C) Hela cells were treated with 25 μg/ml RGD-hylin a1-HMS (pH = 5.5) for 24 h; (D) RGD-hylin a1-HMS induced apoptosis of Hela cells. Hela cells were treated with various concentrations of RGD-hylin a1-HMS (pH = 7) for 24 h. (E) RGD-hylin a1-HMS induced apoptosis of Hela cells. Hela cells were treated with various concentrations of RGD-hylin a1-HMS (pH = 5.5) for 24 h. (F) Quantification of apoptosis rates of HMS-COOH, RGD-hylin a1, and RGD-hylin a1-HMS (pH = 7 and pH = 5.5) in Hela cells.