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. 2017 Sep 11;7:11183. doi: 10.1038/s41598-017-10694-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation and identification of EVs secreted from H69 cells. (a) The morphology of PBS- and LPS-derived EVs analyzed by transmission electron microscope. Scale bar: 100 nm. (b) Nanoparticle tracking analysis for isolated H69-derived EVs. EV concentrations were calculated depending on the particle size. An example for PBS- (solid line) and LPS-derived (dashed line) EVs is shown (left). Total EV concentrations for PBS- and LPS-derived EVs (n = 6, right). (c) Immunoblotting for EV markers. Total H69 cell lysates and H69-derived EV lysates were analyzed by SDS-PAGE. The position of molecular weight markers for each protein is shown.