Table 2.
Genotype frequencies of DNMTs genetic variants among the cases and controls and their association with male infertility.
| SNPs | Genotype | Control (n = 410)a | Case Group I (n = 191)b | Case Group II (n = 642)c | ||||
|---|---|---|---|---|---|---|---|---|
| n (%) | n (%) | P d,e | OR (95% CI)d,f | n (%) | P d,e | OR (95% CI)d,f | ||
| rs4804490 | CC | 144 (35.3) | 55 (29.3) | 1.00 (reference) | 204 (31.9) | 1.00 (reference) | ||
| CA | 166 (40.7) | 96 (51.1) | 0.048 | 1.50 (1.00–2.25) | 324 (50.6) | 0.023 | 1.40 (1.05–1.86) | |
| AA | 98 (24.0) | 37 (19.7) | 0.985 | 1.00 (0.61–1.63) | 112 (17.5) | 0.281 | 0.82 (0.58–1.17) | |
| C-allele carriers | 310 (76.0) | 151 (80.3) | 1.00 (reference) | 528 (82.5) | 1.00 (reference) | |||
| AA | 98 (24.0) | 37 (19.7) | 0.294 | 0.79 (0.52–1.22) | 112 (17.5) | 0.012 | 0.68 (0.50–0.92) | |
| rs1550117 | GG | 263 (64.8) | 115 (60.2) | 1.00 (reference) | 405 (64.0) | 1.00 (reference) | ||
| GA | 122 (30.0) | 70 (36.6) | 0.163 | 1.30 (0.90–1.89) | 201 (31.8) | 0.829 | 1.03 (0.78–1.36) | |
| AA | 21 (5.2) | 6 (3.1) | 0.452 | 0.69 (0.27–1.80) | 27 (4.3) | 0.476 | 0.80 (0.44–1.46) | |
| G-allele carriers | 385 (94.8) | 185 (96.9) | 1.00 (reference) | 606 (95.7) | 1.00 (reference) | |||
| AA | 21 (5.2) | 6 (3.1) | 0.315 | 0.62 (0.24–1.58) | 27 (4.3) | 0.457 | 0.80 (0.44–1.44) | |
| rs2424909 | CC | 344 (86.4) | 160 (83.8) | 1.00 (reference) | 537 (84.8) | 1.00 (reference) | ||
| CT | 46 (11.6) | 30 (15.7) | 0.246 | 1.35 (0.81–2.23) | 93 (14.7) | 0.255 | 1.25 (0.85–1.83) | |
| TT | 8 (2.0) | 1 (0.5) | 0.242 | 0.29 (0.04–2.32) | 3 (0.5) | 0.040 | 0.25 (0.06–0.94) | |
| C-allele carriers | 390 (98.0) | 190 (99.5) | 1.00 (reference) | 630 (99.5) | 1.00 (reference) | |||
| TT | 8 (2.0) | 1 (0.5) | 0.229 | 0.28 (0.03–2.25) | 3 (0.5) | 0.034 | 0.24 (0.06–0.90) | |
| rs7354779 | TT | 384 (93.7) | 177 (95.2) | 1.00 (reference) | 597 (94.1) | 1.00 (reference) | ||
| TC | 24 (5.9) | 9 (4.8) | 0.555 | 0.79 (0.35–1.75) | 36 (5.7) | 0.978 | 0.99 (0.58–1.70) | |
| CC | 2 (0.5) | 0 (0) | — | — | 2 (0.3) | 0.673 | 0.65 (0.09–4.73) | |
| T-allele carriers | 408 (99.5) | 186 (100) | 1.00 (reference) | 633 (99.7) | 1.00 (reference) | |||
| CC | 2 (0.5) | 0 (0) | 0.593 | 0.88 (0.55–1.41) | 2 (0.3) | 0.675 | 0.65 (0.09–4.74) | |
SNPs, single-nucleotide polymorphisms. OR, odds ratios; CI, confidence interval.
aProven fertility men with semen volume ≥ 2 ml, sperm concentration ≥20 × 106/ml, sperm number per ejaculum ≥40 × 106/ml and sperm motility ≥50% motile sperm.
bIdiopathic infertile men with all four semen parameters at or above the WHO reference value.
cIdiopathic infertile men with at least one of the abnormal semen parameters (semen volume, sperm concentration, sperm number per ejaculum and sperm motility).
dAdjusted for age, BMI, smoking status and drinking status.
eTwo-sided χ2 test for genotype distributions between cases and controls.
fORs were obtained from multivariate logistic regression analysis.