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. 2017 Sep 11;7:11200. doi: 10.1038/s41598-017-11514-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microbial community profiles in normal term placentas are spatially distinct based on absolute abundance of the 16S gene and alpha-diversity. (A) Schematic of the gestational compartment depicting the maternal side (BP) and fetal side (PV and FM). (B) Copy numbers/µL of validated V4 samples are plotted per sample type—BP (N = 21), PV (N = 13), FM (N = 16), Blank (N = 8), Water (N = 9). Samples above the dotted threshold (>34 Copies/µL) indicate samples that have met stringency filter set at the maximum copy number/uL of the negative controls (BP vs. FM–P = 0.04; BP vs. PV–0.66; FM vs. PV–P = 0.98; Blanks vs. Water–P = 0.17). (C) Shannon diversity score of validated samples grouped by sampling location (FM vs. BP: P = 0.01, PV vs. BP: P = 0.94, PV vs. FM: P = 0.07). Statistical analyses were performed using Mann-Whitney and significant differences are designated with the following annotations: P = *>0.05, *<0.005, *<0.0005.