Table 1.
Chromatographic and spectral characteristics of the separated compounds.
| Compound | UV-vis absorption maxima as determined (nm)a | UV-vis absorption maxima of standards35, 50 (nm) | Rt (min) | Rf | Resonant Raman maxima as determined (cm−1) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cnd (torulene-like) | 461 | 488 | 517 | 461 | 487 | 518 | 11,71 | nd | nd | nd | nd |
| C1 (torularhodin) | 471 | 498 | 526 | 473 | 498 | 525 | 12,79 | 0,46 | 1511 | 1284 | 1149 |
| C2 (torulene) | 461 | 489 | 518 | 460 | 485 | 516 | 14,14 | 0,52 | 1508 | 1283 | 1151 |
| C3 (β-carotene) | 428 | 454 | 477 | 430 | 452 | 478 | 18,8 | 0,92 | 1505 | 1286 | 1150 |
| C4 (ergosterol) | 272 | 282 | 293 | 272 | 282 | 293 | 29,25 | nd | nd | nd | nd |
aThe solvent strongly influences the spectrum profile; therefore, for reference, a solvent with similar polarity had to be used for a proper comparison. nd = not detected.