Figure 2.

Structures of the Dut80α D95E mutant. Dut80α wt and D95E mutant form similar trimers. Two orthogonal ribbon representations of the trimers from Dut80α (A) wt (PDB: 3ZEZ¹³) and D95E (B) Type I and (C) Type II crystals. Asp95 and Glu95 occupying the central part of trimer channel are represent as sticks in the wt and mutant structures, respectively. The Mg ion coordinated by the acidic residues at position 95 is shown as a sphere and labelled. In the structure of trimeric Dut80α wt (individual monomers in different hues of green) motif V (dark-blue) and motif VI (cyan) involved in Stl interaction are highlighted and labelled. Motif VI are also labelled in D95E Type I (monomers y different hues of blue) and Type II (monomers in yellow, orange and salmon) but Motif V is only labelled in Type II since it is only partially visible in this structure. Nucleotides at the active center are shown as sticks and labelled.