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. 2017 Sep 11;7:11196. doi: 10.1038/s41598-017-11627-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The characteristics of target sequences in UMOD gene. Target sequences on UMOD gene CpG island region. (A) The target sequence is located on the CpG island of UMOD gene (location). F stands for forward primer and R stands for reverse primer. (B) Sequencing validation of the MSP product. The top row of the sequences represents the original gene sequence, and the second row shows the converted sequence. (C) The fragment length of MSP product is 73 bp.