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. 2017 Sep 11;7:11144. doi: 10.1038/s41598-017-11661-8

Figure 2.

Figure 2

Primary pancreatic tumor cell lines that lack ATM express Tp53 but not p16 Ink4a/p19 Arf. (A) Heat map showing the expression levels of PDA classifiers19, 20 in cell lines generated from KC, KCATMΔ+ and KCATMΔΔ primary tumors (PDAC) or liver metastases (LM). The color code (right) indicates the range of expression after normalizing with β-actin. (“Pancreas” is from a P7 mouse pancreas). (B) Immunostainig results demonstrate expression of Hnf1β in primary tumors (PDAC) and liver metastases (LM) of all 3 genotypes (Scale bar is 50 µm.) (C) QPCR results demonstrate retention of Tp53 expression in tumor cell lines established from KC, KCATMΔ+ and KCATMΔΔ primary tumors (PDAC) and liver metastases (LM), and loss of p16 Ink4a/p19 Arf expression in the majority of those cells. (Error bars represent ± SEM values of triplicate experiments).