Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 11;7:11148. doi: 10.1038/s41598-017-11718-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

siRNA-mediated downregulation of GFI1 expression in Fujioka cells (A) GFI1 transcript abundance was determined by quantitative-PCR in cells transfected with GFI1 siRNA and negative control siRNA. Results are representative of 5 independent experiments. Statistical significance was calculated using student’s t-test. ***< 0.001, **< 0.01, *< 0.05. (B) Immunoblot analysis of GFI1 protein in extracts from cells transfected with GFI1 siRNA and negative control siRNA. GAPDH was used as internal control. For detection of GFI1 the membrane was cut into two just below the 46 KDa ladder mark to avoid detection of a cross-reacting non-specific band at around 40 KDa. (C) Cell viability was calculated by counting transfected cells every 24 hours for 4 consecutive days. (D) Flow cytometric staining of transfected Fujioka cells with a labelled antibody against FLT3. (E) RNA quantification by q-PCR of FLT3-ITD molecular signature component 24 hours post siRNA transfection in Fujioka cells. Statistical significance was calculated using student’s t-test. ***< 0.001, **< 0.01, *< 0.05.