Figure 7.

NK cells expanded by the combination of the αCD16 mAb with IrAPs have a strong in vivo antitumor effect in lung and colon cancer xenograft models. A549 cells (2 × 10⁶) and SW480 cells (5 × 10⁶) were subcutaneously injected into the right thigh of SCID mice. IR was applied at 4 and 8 Gy to the tumor in the right thigh of the mice. Expanded NK cells (10⁷) were intravenously injected into each mouse. 5-FU (100 mg/kg) or Doc (5 or 10 mg/kg) were administered into the tail vein 3 days before every NK injection. A and B, Tumor volume (length × width² × 0.5) are expressed as the average tumor volume ± SD of five mice per group (A549- and SW480-bearing mice). (C,D) Relative NKG2D ligand expression ratios were calculated by dividing irradiated (C) and chemotherapeutic drug-treated (D) target cell MFI by untreated-target-cell MFI. All experiments performed independently three times. The statistical significance was determined by one-way ANOVA and paired Student’s t test. ^# P < 0.05, ^## P < 0.005, ^### P < 0.0005 (^#Control versus other groups). ***P < 0.0005 (*NK versus other groups). ^@ P < 0.05, ^@@ P < 0.005, ^@@@ P < 0.0005 (^@IR at 8 Gy (Fig. 7A) versus other groups; IR at 4 Gy versus other groups; Doc 5 mg versus other groups). ^※※ P < 0.005 (^※IR at 8 Gy (Fig. 7B) versus NK + IR at 8 Gy). ^$ P < 0.05, (^$NK + IR at 4 Gy versus NK + IR at 8 Gy).