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. 2017 Sep 11;7:11062. doi: 10.1038/s41598-017-11640-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FgATG1 and FgATG5 complement the phenotypic defects of M. oryzae ∆Moatg1 and ∆Moatg5 mutants respectively. (A) Bar chart showing the conidial production of the strains. Error bars in each column represent standard errors of three independent experiments. Different letters in each column indicate the significant difference of conidiation (P < 0.05). (B) Infection assays on the barley leaves. Leaves of 10-day-old barley seedlings were inoculated with mycelial plugs (0.5 cm) and examined at 7 days post inoculation (dpi). The complemented strains (∆Moatg1/FgATG1 and ∆Moatg5/FgATG5) were fully pathogenic to barley leaves.